Regulatory

Part:BBa_I766557:Design

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-08-16)


pSte5 (Weak) Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter comes from the endogenous expression of pSte5 in yeast. Expression under this promoter is low in comparison to the other constitutive promoters used within our project (pAdh1 and pCyc1).

Source

Promoter from the endogenous yeast scaffold protein Ste5 (YDR103W) [ Pheromone-responsive scaffold protein; shuttles from the nucleus to the plasma membrane and assembles kinases Ste11p, Ste7p, and Fus3p into a specific signaling complex; active oligomeric form interacts with Ste4p-Ste18p complex].

References

For more technical information concerning this promoter's origin, visit www.yeastgenome.org and query the gene ID YDR103W for Ste5.